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| Solution Information | help | |
| Enzyme: | cAMP-dependent protein kinase type II-beta regulatory subunit | |
| inhibitor: | BDBM81290 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this biochemical assay is to determine if compounds identified as active in a set of previous experiments entitled, "Fluorescence polarization-based biochemical primary high throughput screening assay to identify activators of the Protein Kinase A-R1A (PKA-R1A) complex" (AID 504707) and that confirmed activity in a set of experiments entitled, "Fluorescence polarization-based biochemical high throughput confirmation assay for activators of the Protein Kinase A-R1A (PKA-R1A) complex" (AID 504800) and that were inactive in a set of experiments entitled, "Counterscreen for activators of the Protein Kinase A-R1A (PKA-R1A) complex: fluorescence polarization-based biochemical high throughput screening assay to identify fluorescence polarization assay artifacts (AID 504799) are non-selective due to activation of PKAR2B. This fluorescent polarization-based assay monitors activation of PKA holoenzyme (10). This assay employs a 20-residue peptide (IP20) ligand c | |
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